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- Streptavidin-Alexa Fluor® 594, Streptavidin-AF594, SAV
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See Alexa Fluor® 594 spectral data
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 405240 | 100 µg | 212⬠| ||||
Description
Streptavidin binds to biotin with high affinity. Streptavidin-Alexa Fluor® 594 is useful for detecting biotinylated antibodies.
Product Details
Product Details
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- Streptavidin was conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
- Storage & Handling
- The streptavidin-Alexa Fluor® 594 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
- ICC -Quality testedIHC-F -VerifiedIHC-P
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Alexa Fluor® 594 conjugation. For flow cytometric staining, the suggested use of this reagent is ⤠0.125 µg per million cells in 100 µL volume.ÂFor immunohistochemistry on frozen tissue sections, a concentration range of 2.5Â- 5.0µg/mLÂis suggested.ÂIt is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue⢠are trademarks of Life Technologies Corporation.View full statement regarding label licenses
- Application Notes
Streptavidin-Alexa Fluor® 594 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Alexa Fluor® 594 is 60 kD and Streptavidin alone is 52 kD.
- Application References
(PubMed link indicates BioLegend citation)
-
- Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245.
- Nishimoto KP,et al. 2008. J. Immunol. 181:4010. PubMed
- Niki T, et al. 2009. J. Biol. Chem. 284:32344. PubMed
- Shibui A, et al. 2011. Exp Parasitol. 129:318. PubMed
- Scatizzi JC, et al. 2012. J. Immunol. 188:3307. PubMed
- Yamakawa N, et al. 2012. Int Immunol. PubMed
- Shibata T, et al. 2012. Int Immunol. 24:613. PubMed
- Baccala R, et al. 2012. J. Immunol. 189:5976. PubMed
- Grevers LC, et al. 2013. Ann Reheum Dis. 72:278. PubMed
- Woo SJ, et al. 2013. J Leukoc Biol. 93:363. PubMed
- Ashbaugh JJ, et al. 2013. J. Immunol. 190:4525. PubMed
- Reading JL, 2013. J. immunol. 190:4542. Pubmed
- Kanno A, et al. 2013. Int Immunol. 25:413. PubMed
- Gunaydin G, et al. 2014. Vaccine. 32:470. PubMed
- Product Citations
-
- Cheng WH, et al. 2023. Int J Mol Sci. 24:. PubMed
- Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
- Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed
- Thuault S, et al. 2020. Sci Rep. 10:6787. PubMed
- Chen C, et al. 2021. J Virol. 95:e0082921. PubMed
- Fujii T, et al. 2021. Bone Res. 9:4. PubMed
- Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
- Soukupova M, et al. 2021. Mol Ther Methods Clin Dev. 21:399. PubMed
- Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
- Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
Antigen Details
- Gene ID
- NA
- UniProt
- View information about Biotin on UniProt.org
Documentation
- Technical Data Sheet (pdf)
- Certificate of Analysis
- Safety Data Sheet
Reviews
Review This Product
Related Protocols
- Immunohistochemistry Protocol for Frozen Sections
- Immunocytochemistry Staining Protocol
Related Products
| Description | Clone | Applications |
|---|
Pages
- Quality Control
- Alexa Fluor® 594
- Multicolor Staining Guide
- Fluorescence Spectra Analyzer
- Streptavidin Conjugates
- Fluorophore Brightness Index
- Custom Panel Construction
- Fluorophore Equivalency Tool
Related FAQs
There are no FAQs for this product.
Certificate of Analysis
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For Research Use Only. Not for diagnostic or therapeutic use.
This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for userâs intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.
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